DNA digestion to deoxyribonucleoside: A simplified one-step procedure
نویسندگان
چکیده
منابع مشابه
Simplified mammalian DNA isolation procedure.
The reverse genetics technologies that have recently been developed for mice have provided new tools to probe gene function in vivo. Unfortunately these powerful systems often require the analysis of large numbers of DNA samples. The genetargeting technology requires screening of embryonic stem-cell clones and later of the mice themselves, the latter also being the case for standard transgenic ...
متن کاملTechnical note: a modified three-step in vitro procedure to determine intestinal digestion of proteins.
An in vitro, batch incubator (Daisy(II)) was used to simplify the 3-step, in vitro procedure (TSP) to reduce the cost and labor involved in the determination of intestinal digestion of proteins. Four tests were conducted to study the effects of the type of pepsin (P-7012 and P-7000; Sigma, St. Louis, MO), the type of bags used for the incubation of samples (R510 and F57; Ankom Technology, Fairp...
متن کاملA simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated ...
متن کاملSimplified gene synthesis: a one-step approach to PCR-based gene construction.
PCR-based gene synthesis conventionally requires two steps: first, all overlapping oligonucleotides are assembled by self-priming; then an additional pair of primers is used to amplify the full-length gene product. Here we propose a simplified method of gene synthesis which combines these two steps into one. We have found that the efficiency of this one-step method, which we term "Simplified Ge...
متن کاملA three-step in vitro procedure for estimating intestinal digestion of protein in ruminants.
A three-step in vitro procedure was developed to estimate intestinal digestion of proteins in ruminants. Dacron bags containing feed samples were suspended in the rumen for 16 h. Residue containing 15 mg of N after ruminal exposure was incubated for 1 h in 10 mL of a .1 N HCl solution containing 1 g/L of pepsin. After incubation, pH was neutralized with .5 mL of 1 N NaOH and 13.5 mL of a pH 7.8...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2008
ISSN: 0003-2697
DOI: 10.1016/j.ab.2007.09.031